By Yonggang Ke, Pengfei Wang
This exact quantity provides a complete technical review of DNA nanotechnology with an emphasis on 3D DNA nanostructure layout and functions. insurance spans from simple layout ideas for DNA and RNA nanostructures to their state-of-the-art purposes in various fields, with the ebook divided into uncomplicated DNA and RNA nanostructure layout concepts in addition to functions using DNA nanostructures, together with yet now not constrained to nanomedicine, bioimaging, biosensing, nanoplasmonics, nanoelectronics, nanofabrication, crystallography, biophysics, and analytical chemistry. Written for the hugely winning Methods in Molecular Biology sequence, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and tips about troubleshooting and heading off identified pitfalls.
Comprehensive and authoritative, 3D DNA Nanostructure: tools and Protocols presents the main up to date instructional variety overviews and technical kind protocols to profit researchers in a wide selection of areas.
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Extra info for 3D DNA Nanostructure: Methods and Protocols
This DNA brick approach uses synthetic short single-stranded oligomers that interact with one another though an eight nucleotide (nt) binding domain. The DNA brick method provides an attractive alternative for designing structures because of its ease of use, scalability, and modularity. 4 MDa to as large as 8 MDa. The modularity allows for facile design of complex 3D shapes by simply choosing to include or removing specific strands in a given canvas structure. In this chapter, we discuss the design of the discrete and periodic DNA brick structures from strand architecture, shape design, sequence specification, structure annealing, and structure characterization.
Add 3 μL of concentrated sample on the TEM grid, start the blotting process and plunge the grid into the liquid ethane. Transfer the grid from the liquid ethane to a grid box in the liquid nitrogen quickly. Store the grid in a liquid nitrogen dewar. 4. Collect the cryoEM images using a Gatan 4K × 4K CCD camera or by films on a FEI CM200 TEM with accelerating voltage of 200 kV under low-dose condition to minimize radiation damages to the samples. To enhance the image contrast, under-focus in the range of 2–4 μm is used to record the images.
Seeman NC (2010) Nanomaterials based on DNA. Annu Rev Biochem 79:65–87 2. Lin CX, Liu Y, Rinker S, Yan H (2006) DNA tile based self-assembly: building complex nanoarchitectures. Chemphyschem 7:1641–1647 3. Aldaye FA, Palmer AL, Sleiman HF (2008) Assembling materials with DNA as the guide. Science 321:1795–1799 4. Chen JH, Seeman NC (1991) Synthesis from DNA of a molecule with the connectivity of a cube. Nature 350:631–633 5. Zhang YW, Seeman NC (1994) Construction of a DNA-truncated octahedron.
3D DNA Nanostructure: Methods and Protocols by Yonggang Ke, Pengfei Wang