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Download e-book for iPad: Biotechnology, Bioprocessing by G. Stephanopoulos

By G. Stephanopoulos

ISBN-10: 1560811536

ISBN-13: 9781560811534

ISBN-10: 1560816023

ISBN-13: 9781560816027

ISBN-10: 3527283102

ISBN-13: 9783527283101

ISBN-10: 3527283137

ISBN-13: 9783527283132

Bioprocessing: a thrilling new engineering self-discipline. It combines the advance and optimization of biotechnological techniques with potent techniques to recuperate and purify the specified items. safeguard in addition to price play a major position right here. This quantity covers the immensely differentiated spectrum of strategies and operations of bioprocessing, provided by means of the main useful specialists within the box. an outline of upstream and downstream processing is given, fermentation and telephone tradition methods and the layout of microbial fermenters are awarded. A last team of chapters is devoted to problems with method validation, size, and law. issues incorporated are: business mobile Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen move/ Scale Implications/ Fermentation facts research/ cellphone and particles elimination/ Protein Purification/ Electrokinetic Separations/ ultimate restoration Steps/ method Validation

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Read e-book online Biotechnology, Bioprocessing PDF

Bioprocessing: an exhilarating new engineering self-discipline. It combines the advance and optimization of biotechnological approaches with powerful options to get better and purify the specified items. security in addition to expense play a major function the following. This quantity covers the immensely differentiated spectrum of strategies and operations of bioprocessing, awarded via the main efficient specialists within the box.

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1988). The slow growth of animal cells raises concern over contamination 30 2 Industrial Animal Cell Culture by microbes, as well as infection with mycoplasma. The genetic stability of the host cell must be validated by comparison of isoenzyme patterns and immunological markers on samples from the beginning to the end of the production run. Viral particles must be removed to prevent their carry over into the product; this is validated through “spiking” experiments measuring the clearance of each downstream processing step.

Perfusion operations retain cells within the reactor while allowing a cell-free sidestream to be removed; they can be subdivided into two categories, the homogeneous systems such as the perfusion chemostat or heterogeneous systems like hollow fiber or fluidized bed bioreactors. In a perfusion chemostat, cells are continuously recycled from the product stream either by an internal spin filter or via external tangential flow filtration. 3 Operating Strategies state cell viability. In the heterogeneous systems cells are immobilized or entrapped on a biocompatible surface thus preventing them Bioreactor operation strategies can be clas- from exiting in the product stream.

1980), Preparation, sterilization and design of media, in: Fungal Biotechnology (SMITH,J. , BERRY,D. ), pp. 25-41, New York: Academic Press. , OGAWA,M. (1992), “Lights Out” production of cephamycins in automated fermentation facilities, in: Harnessing Biotechnology for the 21st Century (LADISCH,M. ), pp. 223-227, Washington, DC: American Chemical Society. EINSELE,A. , July, 13-14. , PETRE, J. FEHRENBACH, 0. (1992), On-line biomass monitoring by capaciAcknowledgements tance measurement, J. Biotechnol.

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Biotechnology, Bioprocessing by G. Stephanopoulos

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